When using transmissive excitation, blue light can be used to adjust the illumination system according to common brightfield illumination methods, including adjusting the light source and adjusting the center of the condenser. If you use a darkfield condenser, you also need to pay attention to the use and adjustment methods of the darkfield condenser. After adjusting the blue light, the color filter can be changed to obtain the desired excitation light to illuminate the specimen, but note that the diaphragm stop is already in the light path to protect the observer’s eyes from injury. UV damage.

When using reflective excitation, first add a green excitation filter to the optical path, fully open the illumination variable aperture diaphragm and field diaphragm, and adjust the position of the light source so that the illumination beam passes through the center of the screw hole in the spot. The objective lens and make it the source of the image on the object plane. At this time, the screw hole of the objective lens should be a through hole. Check that the excitation beam passes through the center of the objective screw hole. You can place translucent paper on the end face of the screw hole to see if the spot is centered. This is achieved by moving the condenser along the axis.

When installing the light source, be careful not to contaminate the surface of the light source with fingerprints or other marks, which may be difficult to clean after ignition. Therefore, when installing the light source, the surface of the headlight housing can be wiped clean with gauze dipped in organic solvent such as alcohol. It is also necessary to pay attention to the direction of the electrodes and install them according to the requirements of the light source to avoid accidents or ignition failures. Before lighting the tube, it should be placed in the light box to prevent explosion and injury. The combination of excitation filter and suppression filter should be selected according to the excitation method of different fluorescent samples, and usually can also be selected according to the support method recommended in the instruction manual.