1. When using an optical microscope, everyone should pay attention to whether the overall installation of the microscope is loose. For example, if the optical microscope is used for a long time, it will cause problems such as loosening of the bracket and loose installation of the objective lens. If it is too loose, it will damage the actual effect of the inspection, and it is easy to damage the optical microscope.

2. Common problems with using the quasi-focusing helix. It is easy for everyone to focus immediately at high magnification throughout the operation, or both eyes are looking at the eyepieces the entire time whether the barrel is raised or lowered, or the critical point of object distance is not grasped, when the object When the distance is adjusted to 2~3 minutes, it will rise, and the speed of the quasi-focusing spiral is very fast. Such problems can easily cause the objective lens to be reversed and damage the loading or lens. In view of the above practical operation problems, what the editor wants to say is that when adjusting the focal length of the lens, you must first rotate the coarse focus screw under the low magnification lens, so that the lens barrel is gradually lowered, and the objective lens is close to the cover glass, but be careful not to Let the objective lens touch the cover glass. During the entire procedure, both eyes are looking at the objective from the side, then the eyepiece with the right eye, and gradually adjust the coarse quasi-focusing screw in the opposite direction, so that the lens barrel slowly rises until a virtual image is seen. In addition, trainees were shown that the object distance of an ordinary optical microscope is about 1 cm. Therefore, if the distance of the object is much greater than 1cm, but no virtual image is seen, the specimen collection may not be seen, or the coarse quasi-focusing spiral is rotating too fast. The loading position should be adjusted at this point and the above process repeated. When blurry virtual images appear in the field of view, it is necessary to switch to precise quasi-focus screw adjustment. Only in this way can you narrow down your search and increase the speed of searching for virtual images.

3. Difficulties with light in optical microscopy. This is an important step in the application of light microscopy to light. Remember, you have to use low powered mirrors for lighting. When the light source is strong, use a small focal length plane mirror, and when the light source is poor, use a large aperture lens and a concave mirror. When you turn the mirror, you can’t pull it out with one hand. You have to rotate it with both hands until you see a symmetrical bright circular sight. After the light is aligned, do not move the optical microscope at will, in case Confucius illuminates the rearview mirror and the light source cannot enter accurately.

4. The difficulty of objective lens transformation. When using a low-power mirror, switch to a high-power mirror, everyone usually likes to put the objective lens immediately with a finger, which is more economical, but it is easy to tilt the optical axis of the objective lens, because the raw material of the converter is too soft in color, high precision, non- Symmetrical male threaded brackets come loose easily. Once the outer thread is destroyed, all converters will be charged. Therefore, the editor of the website recommends that you hold the lower turntable of the converter to change the objective lens.

5. The problem of using the eye to do a high power microscope. When everyone is using a high power microscope block, they should break their eyes, stare into the eyepiece, that is, the right eye should be as close as possible to the eyepiece, and try to stare without forcibly covering the right eye or closing the right eye decisively . Do not meet the observation requirements of the test, often the right eye is easily fatigued, and it cannot be guaranteed to be extracted during observation and inspection.